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cdna synthesis kit  (TaKaRa)


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    Structured Review

    TaKaRa cdna synthesis kit
    Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 6093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cdna+synthesis+kit/SMARTer+PCR+cDNA+Synthesis+Kit/pmc13655257-442-6-9
    Average 96 stars, based on 6093 article reviews
    cdna synthesis kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: Clinical significance of serum miR-95 in children with Mycoplasma pneumoniae infection complicated with diarrhea.
    Article Snippet: Total RNA was isolated using Trizol reagent (Invitrogen). .. Subsequently, 1 μg RNA underwent reverse transcription with a commercial cDNA synthesis kit (Takara). .. RT-qPCR was performed on an ABI 7500 RealTime PCR System (Applied Biosystems) employing SYBR Premix Ex Taq reagents (Takara), with all samples analyzed in triplicate biological replicates.

    Article Title: A Dual‐Delivery System Designed to Overcome EV‐Mediated Ferroptosis Resistance for Efficient Solid Tumor Therapy
    Article Snippet: Total RNA was extracted using RNAiso reagent (Takara). .. Reverse transcription was achieved using a cDNA Synthesis Kit (Takara). .. mRNA levels were measured using gene‐specific primers and SYBR Premix Ex TaqTM kits (Takara).

    Article Title: HIPK2 exhibits context dependent dualistic effects and combinatorial inhibition of HIPK2-NLK-MAPK11 axis manifests synergism against gastric cancer.
    Article Snippet: Homeodomain-interacting protein kinase 2 (HIPK2) can function either as a tumour suppressor or promoter in cancer.. Understanding key features of HIPK2 context dependent effects in cancer could lead to novel therapeutic strategies.. Our investigation of HIPK2 expression in gastric cancer (GC) showed it to be down regulated.HIPK2 manifested context dependent antiand pro-tumorigenic effects as demonstrated in GC cell lines SNU638 and NUGC-3 (β-Cateninlow, CD44Hi) and AGS (β-CateninHi, CD44low).

    Article Title: Association of miR-10a expression and its rs3809783 polymorphism with postoperative incisional pain via Bcl-6/Tfh targeting in patients undergoing laparoscopic cholecystectomy
    Article Snippet: .. Total RNAs were reverse transcribed into cDNAs using a cDNA Synthesis Kit (TaKaRa, Osaka, Japan), and the cDNAs were amplified using the SYBR Premix Ex Taq II Kit (TaKaRa, Osaka, Japan). .. miR-10a levels were measured using the LightCycle 96 Real-time PCR System (Roche, Basel, Switzerland).

    cDNA Synthesis:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Article Title: Clinical significance of serum miR-95 in children with Mycoplasma pneumoniae infection complicated with diarrhea.
    Article Snippet: Total RNA was isolated using Trizol reagent (Invitrogen). .. Subsequently, 1 μg RNA underwent reverse transcription with a commercial cDNA synthesis kit (Takara). .. RT-qPCR was performed on an ABI 7500 RealTime PCR System (Applied Biosystems) employing SYBR Premix Ex Taq reagents (Takara), with all samples analyzed in triplicate biological replicates.

    Article Title: Panax -derived Zingibroside R1 exerts allelopathic effects on root architecture by modulating the auxin transport pathway and oxidative stress responses
    Article Snippet: RNA integrity and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. First-strand cDNA synthesis was performed with 0.5 μg of total RNA using a cDNA Synthesis Kit (Takara, Japan), followed by second-strand cDNA synthesis employing DNA polymerase I, dNTPs, and RNase H. The resulting double-stranded cDNA was purified using a PCR extraction kit and processed through end repair, A-tailing, and adapter ligation to construct sequencing libraries. .. High-throughput sequencing was conducted on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA).

    Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells.
    Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS.

    Article Title: A Dual‐Delivery System Designed to Overcome EV‐Mediated Ferroptosis Resistance for Efficient Solid Tumor Therapy
    Article Snippet: Total RNA was extracted using RNAiso reagent (Takara). .. Reverse transcription was achieved using a cDNA Synthesis Kit (Takara). .. mRNA levels were measured using gene‐specific primers and SYBR Premix Ex TaqTM kits (Takara).

    Article Title: Metabolic boosting via mitochondrial transfer for enhanced cell-based wound healing therapy
    Article Snippet: .. For analysis of metabolic gene expressions, RNA was isolated from each sample, 24 h after treatment with a predetermined concentration of mitochondria using an RNA extraction kit (Takara Bio), from which cDNA was synthesized using a cDNA synthesis kit (Takara Bio). .. The RT-qPCR procedure was performed with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Waltham, MA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    Article Title: HIPK2 exhibits context dependent dualistic effects and combinatorial inhibition of HIPK2-NLK-MAPK11 axis manifests synergism against gastric cancer.
    Article Snippet: Homeodomain-interacting protein kinase 2 (HIPK2) can function either as a tumour suppressor or promoter in cancer.. Understanding key features of HIPK2 context dependent effects in cancer could lead to novel therapeutic strategies.. Our investigation of HIPK2 expression in gastric cancer (GC) showed it to be down regulated.HIPK2 manifested context dependent antiand pro-tumorigenic effects as demonstrated in GC cell lines SNU638 and NUGC-3 (β-Cateninlow, CD44Hi) and AGS (β-CateninHi, CD44low).

    Article Title: Association of miR-10a expression and its rs3809783 polymorphism with postoperative incisional pain via Bcl-6/Tfh targeting in patients undergoing laparoscopic cholecystectomy
    Article Snippet: .. Total RNAs were reverse transcribed into cDNAs using a cDNA Synthesis Kit (TaKaRa, Osaka, Japan), and the cDNAs were amplified using the SYBR Premix Ex Taq II Kit (TaKaRa, Osaka, Japan). .. miR-10a levels were measured using the LightCycle 96 Real-time PCR System (Roche, Basel, Switzerland).

    Quantitative RT-PCR:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Breeding of a higher producer enables identification of a negative transcription factor controlling the biosynthesis of xiamenmycin in Streptomyces xiamenensis
    Article Snippet: .. Reverse transcription was performed using a cDNA synthesis kit (Takara, Japan). qRT-PCR was performed on a StepOnePlus real-time PCR system (Applied Biosystems, USA) using TB GreenTM Premix Ex TaqTM (Takara, Japan). ..

    Purification:

    Article Title: Panax -derived Zingibroside R1 exerts allelopathic effects on root architecture by modulating the auxin transport pathway and oxidative stress responses
    Article Snippet: RNA integrity and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. First-strand cDNA synthesis was performed with 0.5 μg of total RNA using a cDNA Synthesis Kit (Takara, Japan), followed by second-strand cDNA synthesis employing DNA polymerase I, dNTPs, and RNase H. The resulting double-stranded cDNA was purified using a PCR extraction kit and processed through end repair, A-tailing, and adapter ligation to construct sequencing libraries. .. High-throughput sequencing was conducted on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA).

    Polymerase Chain Reaction:

    Article Title: Panax -derived Zingibroside R1 exerts allelopathic effects on root architecture by modulating the auxin transport pathway and oxidative stress responses
    Article Snippet: RNA integrity and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. First-strand cDNA synthesis was performed with 0.5 μg of total RNA using a cDNA Synthesis Kit (Takara, Japan), followed by second-strand cDNA synthesis employing DNA polymerase I, dNTPs, and RNase H. The resulting double-stranded cDNA was purified using a PCR extraction kit and processed through end repair, A-tailing, and adapter ligation to construct sequencing libraries. .. High-throughput sequencing was conducted on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA).

    Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells.
    Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS.

    Extraction:

    Article Title: Panax -derived Zingibroside R1 exerts allelopathic effects on root architecture by modulating the auxin transport pathway and oxidative stress responses
    Article Snippet: RNA integrity and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. First-strand cDNA synthesis was performed with 0.5 μg of total RNA using a cDNA Synthesis Kit (Takara, Japan), followed by second-strand cDNA synthesis employing DNA polymerase I, dNTPs, and RNase H. The resulting double-stranded cDNA was purified using a PCR extraction kit and processed through end repair, A-tailing, and adapter ligation to construct sequencing libraries. .. High-throughput sequencing was conducted on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA).

    Adapter Ligation:

    Article Title: Panax -derived Zingibroside R1 exerts allelopathic effects on root architecture by modulating the auxin transport pathway and oxidative stress responses
    Article Snippet: RNA integrity and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. First-strand cDNA synthesis was performed with 0.5 μg of total RNA using a cDNA Synthesis Kit (Takara, Japan), followed by second-strand cDNA synthesis employing DNA polymerase I, dNTPs, and RNase H. The resulting double-stranded cDNA was purified using a PCR extraction kit and processed through end repair, A-tailing, and adapter ligation to construct sequencing libraries. .. High-throughput sequencing was conducted on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA).

    Construct:

    Article Title: Panax -derived Zingibroside R1 exerts allelopathic effects on root architecture by modulating the auxin transport pathway and oxidative stress responses
    Article Snippet: RNA integrity and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. First-strand cDNA synthesis was performed with 0.5 μg of total RNA using a cDNA Synthesis Kit (Takara, Japan), followed by second-strand cDNA synthesis employing DNA polymerase I, dNTPs, and RNase H. The resulting double-stranded cDNA was purified using a PCR extraction kit and processed through end repair, A-tailing, and adapter ligation to construct sequencing libraries. .. High-throughput sequencing was conducted on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA).

    Sequencing:

    Article Title: Panax -derived Zingibroside R1 exerts allelopathic effects on root architecture by modulating the auxin transport pathway and oxidative stress responses
    Article Snippet: RNA integrity and concentration were assessed using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific, USA). .. First-strand cDNA synthesis was performed with 0.5 μg of total RNA using a cDNA Synthesis Kit (Takara, Japan), followed by second-strand cDNA synthesis employing DNA polymerase I, dNTPs, and RNase H. The resulting double-stranded cDNA was purified using a PCR extraction kit and processed through end repair, A-tailing, and adapter ligation to construct sequencing libraries. .. High-throughput sequencing was conducted on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA).

    SYBR Green Assay:

    Article Title: β-Thujaplicin modulates the ODC-ROS-linked apoptosis in H460 lung cancer cells.
    Article Snippet: This study explored the antiproliferative effects of β-thujaplicin, a natural tropolone derivative, in the non-small-cell lung cancer (NSCLC) cell lines H460 and A549 and in L132 normal lung epithelial cells, using MTT, NRU, and SRB assays.. β-Thujaplicin exhibited concentration-dependent cytotoxicity in H460 and A549 cells but had minimal effect on L132 cells.. Mechanistic studies in H460 cells showed suppression of ornithine decarboxylase (ODC) activity and ODC mRNA expression, along with alterations in polyamine metabolite abundance detected by LC-HRMS.

    Analysis:

    Article Title: Metabolic boosting via mitochondrial transfer for enhanced cell-based wound healing therapy
    Article Snippet: .. For analysis of metabolic gene expressions, RNA was isolated from each sample, 24 h after treatment with a predetermined concentration of mitochondria using an RNA extraction kit (Takara Bio), from which cDNA was synthesized using a cDNA synthesis kit (Takara Bio). .. The RT-qPCR procedure was performed with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Waltham, MA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    Isolation:

    Article Title: Metabolic boosting via mitochondrial transfer for enhanced cell-based wound healing therapy
    Article Snippet: .. For analysis of metabolic gene expressions, RNA was isolated from each sample, 24 h after treatment with a predetermined concentration of mitochondria using an RNA extraction kit (Takara Bio), from which cDNA was synthesized using a cDNA synthesis kit (Takara Bio). .. The RT-qPCR procedure was performed with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Waltham, MA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    Concentration Assay:

    Article Title: Metabolic boosting via mitochondrial transfer for enhanced cell-based wound healing therapy
    Article Snippet: .. For analysis of metabolic gene expressions, RNA was isolated from each sample, 24 h after treatment with a predetermined concentration of mitochondria using an RNA extraction kit (Takara Bio), from which cDNA was synthesized using a cDNA synthesis kit (Takara Bio). .. The RT-qPCR procedure was performed with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Waltham, MA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    RNA Extraction:

    Article Title: Metabolic boosting via mitochondrial transfer for enhanced cell-based wound healing therapy
    Article Snippet: .. For analysis of metabolic gene expressions, RNA was isolated from each sample, 24 h after treatment with a predetermined concentration of mitochondria using an RNA extraction kit (Takara Bio), from which cDNA was synthesized using a cDNA synthesis kit (Takara Bio). .. The RT-qPCR procedure was performed with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Waltham, MA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    Synthesized:

    Article Title: Metabolic boosting via mitochondrial transfer for enhanced cell-based wound healing therapy
    Article Snippet: .. For analysis of metabolic gene expressions, RNA was isolated from each sample, 24 h after treatment with a predetermined concentration of mitochondria using an RNA extraction kit (Takara Bio), from which cDNA was synthesized using a cDNA synthesis kit (Takara Bio). .. The RT-qPCR procedure was performed with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Waltham, MA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    Amplification:

    Article Title: Association of miR-10a expression and its rs3809783 polymorphism with postoperative incisional pain via Bcl-6/Tfh targeting in patients undergoing laparoscopic cholecystectomy
    Article Snippet: .. Total RNAs were reverse transcribed into cDNAs using a cDNA Synthesis Kit (TaKaRa, Osaka, Japan), and the cDNAs were amplified using the SYBR Premix Ex Taq II Kit (TaKaRa, Osaka, Japan). .. miR-10a levels were measured using the LightCycle 96 Real-time PCR System (Roche, Basel, Switzerland).



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